Solidphase peptide synthesis (SPPS) remains the most efficient approach for the chemical preparation of standard peptides. Our peptide chemists team has many years experience in peptide synthesis of various length,
► Standard sequence length: 5 to 30 aa.
► Routine sequence length from 31 to 60 aa.
► Challenging sequence length from 61 to 100 aa.
Because peptides of length over 40 aa have raised important research interests, our customers’ request for long peptides (40 aa up to 80-100 amino acids) is increasing. While being challenging, such large molecules could be successfully synthesized at Genosphere Biotechnologies by stepwise solidphase peptide synthesis.
For example, dipeptide repeats have been implicated in a number of disorders. We have successfully prepared many long to very long direpeats, including proline-containing poly(Pro-lys)20 that have been associated with amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD).
The expansion of GGGGCC repeats within the first intron of C9ORF72 constitutes the most common cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Through repeat-associated non-ATG translation, these expansions are translated into dipeptide repeats (DPRs), some of which accumulate at nucleoli and lead to cell death.
Importantly, the two dipeptide repeats (DPRs) that contain arginine, poly(GR) and poly(PR), are toxic to cells even when added to culture media as well as in model organisms, providing a direct model to explain the pathogenicity of C9ORF72 mutations. Arginine-containing DPRs enter into cells, where they concentrate at nucleoli altering their size and several nucleolus-related functions such as rRNA biogenesis or mRNA splicing, ultimately leading to cell death.